Hello,
I hope to collect samples from lava tubes (microbial mats on the walls and nearby soils) to perform a metagenomics analysis. I will...
21 results in 0.0023 seconds.
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Space/microgravity simulation: Extract DNA for MinION
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Why is my DNA concentration and absorbance ratios fluctuating?
I have done DNA extraction on human saliva using Promega ReliaPrep™ Blood gDNA Miniprep System that have been expired for more than five years now.... -
kpet started a topic does heat-treatment (for covid inactivation) of Oragene saliva DNA kits alter DNA metin Epigeneticsdoes heat-treatment (for covid inactivation) of Oragene saliva DNA kits alter DNA met
Has anyone heat-treated Oragene DNA saliva collection kits and done downstream DNA methylation analysis on the extracted DNA? Any evidence of an effect... -
does the Oragene saliva kit lyse coronavirus?
Does anybody know if the Oragene DNA saliva collection kits(s) lyse coronavirus? -
DNA extraction from buccal cells - any favourite methods?
I'm looking into methods for extracting DNA from buccal cells. Does anyone do this? What method to do you use and why do you use it?
Thanks! -
can I use standard plastic falcon tubes for chloroform DNA extraction?
Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with... -
Bio-On-Magnetic-Beads (BOMB): Open platform for HT nucleic acid extraction
Hi All,
I was thinking this might be interesting for some doing RNA/DNA sequencing, and library preps. We have established home-made methods for... -
Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extracti
Hi All,
I was thinking this might be interesting for some doing RNA/DNA sequencing. We have established home-made methods for HT RNA/DNA extraction... -
CTAB alternative for carboxyl mag bead gDNA extraction
Last summer I developed a protocol for our lab to extract gDNA from tissue samples using the carboxyl coated magnetic beads used in SPRI. It has worked... -
What's the best strategy for sampling to get high molecular weight DNA
Hi all,
there's a lot of chat about extraction tekkers to get HMW DNA so i think we've got that covered; however what i can't find is... -
Optimizing magnetic bead mix for gDNA extraction
I've been working with the protocol in this thread for making magnetic bead mixes: http://seqanswers.com/forums/showthread.php?t=49507
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Please help! DNA samples for whole genome sequencing
Hi folks,
I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit.... -
Advice on DNA Extraction from RNAlater stored tissue
Hi,
I am attempting to extract DNA from RNAlater stored tissue samples for
downstream DNA methylation sequencing (RRBS).
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Bias due to DNA extraction kit and illumine version used
Do you think DNA extraction kit and illumine version used could be a source of variation?
Our idea is to perform genome comparison with bacterial... -
DNA degradation - gill tissue
Hi all,
I was wondering if anybody has experience extracting DNA from fish gills (and the microbes associated with them)? I have been trying...
