Dear all,
in my lab we are using the Nextera Rapid Capture Exome kit to do whole exome sequencing. Until now I have used GRCh37 as a reference...
8 results in 0.0022 seconds.
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align reads generated with Nextera exome kit (designed on GRCh37) to GRCh38?
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Counting Genes Covered in Exome Seq Project
Hi,
I wanted to calculate how many RefSeq genes were covered after QC in a particular Exome Seq project. First, all the bases above a certain coverage... -
Low exomic yeld after capture.
Dear all,
After having used the Targetseq Exome Capture kit for years, LifeTech provided us with a new version of the kit called Ion... -
Agilent SureSelect bed files
Hello,
Can anyone tell me what the difference is between these bed files supplied by Agilent for their SureSelect Human All Exon V2 kit:... -
Necessary oligo modifications for home-made DNA-seq kit
Hi,
I am attempting to put together a set of adapters and blocking oligos for a multiplexed exome-capture experiment. I am following this: ht... -
Sureselect disappearing DNA
Hello All
We are doing the Sureselect protocol (XT all exon V4) with 3ug of DNA, after size selection we have around 1ug left for the end... -
Exome caputure: masking the non-genic sequences before aligning ?
Hi all,
I'm about to map a set of short reads after an exome capture.
I wonder if it would be worth to replace all the... -
Baits length for exome capture? (Nimblegen and Aglient)
Hi guys
Does anyone use NimbleGen and Agilent exome capture?
I wonder how long baits are for two methods.
...
