Header Leaderboard Ad

Collapse

Agilent SureSelect bed files

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Agilent SureSelect bed files

    Hello,

    Can anyone tell me what the difference is between these bed files supplied by Agilent for their SureSelect Human All Exon V2 kit:

    _Regions.bed
    _Padded.bde
    _Covered.bed
    _AllTargets.bed

    Thanks!

  • #2
    The padded bed file is the regions that are baited, with an extension of 50 bases to either end. Where this regions meet they are merged into a single region.

    These tend to extend into the intronic regions (and generally defines what you can confidently genotype).

    Regions is the regions the kit is designed to (I think). Covered is the regions that are baited for capture.

    Comment


    • #3
      Very helpful, thanks.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        How RNA-Seq is Transforming Cancer Studies
        by seqadmin



        Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
        09-07-2023, 11:15 PM
      • seqadmin
        Methods for Investigating the Transcriptome
        by seqadmin




        Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.

        Whole Transcriptome RNA-seq
        Whole transcriptome sequencing...
        08-31-2023, 11:07 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 09-22-2023, 09:05 AM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-21-2023, 06:18 AM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-20-2023, 09:17 AM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-19-2023, 09:23 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Working...
      X