Hello,
I have a data of snatacSeq of 15 batch of patients (5 patients per batch that are multiplexed ), I need to demultplexe them using genotypes...
20 results in 0.0024 seconds.
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Genotyping using Low-Pass Sequencing data (0.5x - 1x)
Hi all, I am currently trying to do some genotyping using some low-pass sequencing data on 13 1000GP samples. I am mostly interested in biallelic SNPS's.... -
What does 'top=?' mean in a FASTA header?
I pipeline I am working with has a step which says:
echo "Rename OTU sequences. All OTU with 'top=?' regarded as 'Other'"
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Haplotyping/genotyping tool PacBio?
Hi all,
we have data from 40 different amplicons of different sizes (400 - 4000 bp) that have various coverage levels sequenced in a plant,... -
gold standard for human genome INDEL calling
Hi all!
I am evaluating the performance of our INDEL calling tool and comparing with others.I am using the INDELs dataset identified by Ryan Mills... -
Genomics and Bioinfromatics job at IMPPC-IGTP - Badalona, Spain
Genomics and Bioinformatics Research Technician - Ref.: 2015/35
Starting date: December 15, 2015.
Salary range: 24.000-30.000... -
RAD-Seq vs genotyping by sequencing
Hello, I'm new to bioinformatics and nextgen sequencing and was wondering if anybody has any major pros and cons on this techinque. Also, the only provider... -
nextRAD publication
We at SNPsaurus quite frequently get asked about publications using nextRAD. Of course, it take a while to go from genotyping data to publication! Today... -
HLA and KIR typing
I've sequenced some HLA and KIR genes by using Agilent SureSelect target enrichment kit, together with other target regions. The sequencing platform was... -
vcf file filtering looking for polimorphisms
Hi everyone:
I recently got my mpileup-bcftools results looking for SNV's on a ~200 sample population. It happens that there is no reference... -
Genotyping service offerings from SNPsaurus
SNPsaurus has new genotyping service offerings with range of options at $49/sample, from performing genome scans of 250,000 nextRAD tags sequenced at... -
Hello from Eric at SNPsaurus
Hi all,
I've been reading SEQanswers for a long time and always been impressed by the community. I'm interested in sequencing methods in... -
2b-RAD? does anyone test it?
Hi all!
Does anyone try to set up a similar experiment?
I'm planning to use 96 tags. Does someone have suggestions about barcodes design?... -
Calling variants in a group of samples sharing a haplotype?
I chose a set of human samples with a common haplotype for illumina resequencing, and targeted the haplotype locus with a capture chip. I would like... -
question for genotying with bcftools
hi,
I am trying to call the genotypes with the mpileup output using bcftools. I found very weird for one SNP, I actually have heterozygous...
