Hi all,
I've been reading SEQanswers for a long time and always been impressed by the community. I'm interested in sequencing methods in general. I am one of the developers of RAD-Seq, which is a genotyping by sequencing method (see http://www.plosone.org/article/info:...l.pone.0003376 for the original article from my lab).
Since then we've developed a new approach to genotyping we are calling nextRAD (nextera-tagmented reductively amplified DNA). It is similar to RAD in that the tags that result are at a fixed location at one end and randomly sheared at the other, giving some advantages for reproducibility. But it doesn't involve restriction enzymes, instead using selective PCR. I think a primary advantage of that is that the input requirements are very low. Depending on the genome, it just needs 1-10ng total. So it may open up some new projects not currently possible because of input requirements. It also seems to be more robust in the face of poor quality DNA compared to restriction-based methods.
Anyway, my lab has a paper coming out about it, and I've started a commercial service provider (SNPsaurus) as well. But I'm generally interested in talking about genotyping and genetics.
I've been reading SEQanswers for a long time and always been impressed by the community. I'm interested in sequencing methods in general. I am one of the developers of RAD-Seq, which is a genotyping by sequencing method (see http://www.plosone.org/article/info:...l.pone.0003376 for the original article from my lab).
Since then we've developed a new approach to genotyping we are calling nextRAD (nextera-tagmented reductively amplified DNA). It is similar to RAD in that the tags that result are at a fixed location at one end and randomly sheared at the other, giving some advantages for reproducibility. But it doesn't involve restriction enzymes, instead using selective PCR. I think a primary advantage of that is that the input requirements are very low. Depending on the genome, it just needs 1-10ng total. So it may open up some new projects not currently possible because of input requirements. It also seems to be more robust in the face of poor quality DNA compared to restriction-based methods.
Anyway, my lab has a paper coming out about it, and I've started a commercial service provider (SNPsaurus) as well. But I'm generally interested in talking about genotyping and genetics.