Hello there,
we consider MBD-seq cause the costs and overall CpG coverage. But I have limited understanding regarding number of reads from Illumina....
20 results in 0.0021 seconds.
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MBD-seq single-end or paired-end - number of reads
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Total RNAseq library - artifact levels up to 95% with clear pattern on 96 well plate
I have (very complex) artifact levels up to 95% in my total RNAseq data.
Some facts:
- NEB Ultra 2 Library prep
- 96 samples per plate,... -
ultralow microRNA library prep
Hi,
Can anyone suggest a library prep kit for illumina hiseq/miseq, which can work with ultralow amount of microRNAs (50 -200 pg) ? or... -
Trimmomatic dropping 100% sRNA reads
Hi All,
This is my first post on SEQanswers and I am hoping some help from senior members . I am analyzing sRNA single end sequencing data... -
454 Sequencers for Eukaryotic Genomes
I would like to know the reason why we don't use 454 Sequencers for for sequencing Eukaryotic Genomes (ChIP-Seq, DNA Seq, RNA-Seq) and why most of the... -
dwgsim to simulate Illumina reads
Hello, I want to use dwgsim to simulate Illumina reads from the 1000 Genomes Project. I am particularly interested in individual NA12878.
... -
High AT dropout
Hi
i am running SCS2.9 on Illumina GAIIx, recently i noticed AT dropout to some of my libraries.All the libraries are made with same procedure,... -
Question concering deletions and inversions inferred from PE-sequencing
Hello everyone,
I've got two questions concerning the detection of structural variations:
1.) We sequenced the same cell line...Last edited by ForeignMan; 05-09-2011, 03:56 AM. -
making a small RNA library from human serum
Hello everyone,
I am trying to sequence microRNAs from human serum using illumina. I have tried sending my sample, total RNA, to NHGRI where... -
Paired end reads: line # does not match
Hello,
I have a GAII paired-end run (seven lanes, run1 and run2 for each lane are the forward and reverse ends of a pair).
For 3... -
Problem with CASAVA (GERALD) - Missing 'chromosome' element
Hi all,
I'm trying to run GERALD with eland_rna analysis and getting the following error:
ERROR: Missing 'chromosome' element... -
Differential analysis of non coding RNA seq
Hello
,
I'm working on ncRNA seq libraries obtained with Illumina... I just start to have a look about statistical analysis between two... -
Ratio in Adapter-Ligation
Dear all,
we design our Adapters and Primers for the Illumina Library by ourselves. Its working very good. The only problem we have is... -
Questions on Agilent SureSelect Indexing Kit
Hi,
does anybody have details on the new SureSelect Indexing Kits?
I am interested in targeting 12 samples and pool and sequence them... -
Insert size with Agilent SureSelect
We have noticed that our Agilent SureSelect libraries have a very small insert size after hybrid selection. Prior to selection, we see BioAnalyzer modes...
