Hi Guys,
So I'm trying to amplify whole mtDNA of a few species using long-range PCR. I've found a conserved region and designed long (30mer)...
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Long Range PCR problem...
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Cleaning amplicons before Nextera + Miseq run??
Hi Guys,
Quick question: I'm planning on sequencing large amplicons (5-15 kbp) on our MiSeq using the Nextera kit. Wondering the best...
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pooling long range PCR
Hello, I have an experiment with 20 over lapping long range PCR fragments that span a single gene. The average size is 5kb (max 10, min 1.5kb) with equimolar...