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SPRI Beads & Magnetic Beads: First-hand Tips
SPRI beads and magnetic beads are well used in molecular labs for nucleic acids purification because they are fast, simple and efficient. The magnetic... -
Low efficiency in bead-based size selection. Can't get rid of large fragments.
Hi everyone!
We’re performing a bead-based size selection of double digested genomic DNA, targeting fragments between 400bp and 700bp,... -
Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extracti
Hi All,
I was thinking this might be interesting for some doing RNA/DNA sequencing. We have established home-made methods for HT RNA/DNA extraction... -
CTAB alternative for carboxyl mag bead gDNA extraction
Last summer I developed a protocol for our lab to extract gDNA from tissue samples using the carboxyl coated magnetic beads used in SPRI. It has worked... -
Optimizing magnetic bead mix for gDNA extraction
I've been working with the protocol in this thread for making magnetic bead mixes: http://seqanswers.com/forums/showthread.php?t=49507
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Aliquoting AMPure XP magnetic beads recomendations
I'm relatively new at working with RNA and I'm getting ready to make my first Library next week. Since I'm new to most these techniques and I want to... -
Miltenyi Microbeads + Nextera DNA Sample Prep
Hi All,
I've been having some trouble purifying my cells by FACS and have been considering moving to the Miltenyi microbead sorting system.... -
Magnetic bead washers
Does anyone know of a automated bead washing machine which will efficiently work with the Ampure purification beads. I don't necessarily want to buy a... -
==> magnetic bead separation magnet
Hello,
during the enrichment step with the OneTouch ES magnetic beads have to be separated. The protocol recommends a DynaMag-2, they are... -
magnetic beads for gel extraction
Hi all,
few months ago we have started to use the magnetic beads for nucleic acid size selecting during SOLiD library preparation.
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