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NEBNext Ultra II Q5 Master Mix
Does anyone know the final "1X" concentration of polymerase in the Q5 Ultra MM in U/uL?
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NEBNext adaptors clustering failed
Hi all,
I am having problems with the NEBnext Ultra kit for library preparation. I´ve been doing same amplicon libraries for three years with...
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crustacean small RNA library QC
Hi, everyone,
We're trying for the first time to prepare small RNA libraries for our crustacean model, and we're using the NEBNext small...
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Size Selection after fragmentation
Hey there,
This is my first post here - thanks for reading it!
I'm rather lost. I'm prepping some samples for RNA-Seq, but...
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NEBNext Adapter Ligation Efficiency
Hello.
Has anyone tried "NEBNext Multiplex Oligos for Illumina" to generate sequencing libraries? I have been experiencing a...
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Kapa vs NEBnext for ChIPSeq?
Hi Seqanswers!
Forgive me if there’s already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does...
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EpMotion - NEBnext ultra program
Was wondering if there is a program out there for genomic library prep on the EpMotion. I know they have the TruSeq RNA one's, but I am more looking...
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RNA fragmentation
Hello all,
I am new to here and RNA-Seq. So I need some help.
I am working on optimizing the RNA fragmentation conditions...
