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Was wondering if there is a program out there for genomic library prep on the EpMotion. I know they have the TruSeq RNA one's, but I am more looking for DNA and hoping specfically for NEBNext Ultra.
Thanks,
A
Thanks,
A
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I know this is an old post but maybe you are still working with this? Any update on a program for library prep with the NEBNext Ultra II FS? I'm having issues with the volumes and the plates to use for it. If you have any pieces of advice I'll be very grateful!Originally posted by acockburn View Post -
Hi,
According to Eppendorf they have a protocol for this procedure: https://www.eppendorf.com/US-en/serv...-for-epmotion/
Might contact them directly.Comment
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NEB Next RNA II
Hello,
I noticed that you're interested in methods for the NEB Next RNA II method. If you would like a copy of these methods, please email Eppendorf's Application Support team. [email protected]
Have a great day,
JesseComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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