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NEB Fragmentase mitogenome advice
Hi, I am currently working with mitogenome sequencing of small mammals. I have amplified 5 PCR amplicons of the mitogenome around 4-5kb fragment lengths....Last edited by frascom; 06-07-2019, 07:26 AM. -
Buy NebNext Ultra DNA Library Kit components individually
Hello everyone,
Can someone explain why the NebNext Ultra DNA Lib Prep Kit is so expensive compared to the sum of the invidivual components?... -
Stingray mRNA Fragmentation NEBNext
Hi all,
I am using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina following mRNA isolation using dT beads.
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Cycle number? Indexing/enrichment NEBNext
I'm preparing dual-indexed libraries using NEBNext Ultra Library Prep for targeted resequencing on Illumina MiSeq and can't decide on the number of PCR... -
Is end repair and 5’phosphate necessary for the adaptor ligation?
Hi,everyone! I’m new here and this forum has helped me a lot,thanks.
We are now hoping to carry out NGS of PCR amplified DNA fragment. The DNA... -
MiSeq Run, Problem with Index reads
I just ran a miseq run using NEBNext Ultra DNA prep, (the bio analyzer results looked fine prior to sequencing).
We sequenced 6 samples...
