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Buy NebNext Ultra DNA Library Kit components individually

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  • Buy NebNext Ultra DNA Library Kit components individually

    Hello everyone,

    Can someone explain why the NebNext Ultra DNA Lib Prep Kit is so expensive compared to the sum of the invidivual components?

    NebNext Ultra DNA Library Prep Kit for Illumina: $535. 24 reactions
    Contains:
    -NEBNext® End Repair Module
    -NEBNext® Q5® Hot Start HiFi PCR Master Mix
    -Blunt/TA Ligase Master Mix
    -NEBNext Ligation Enhacer

    But:
    NEBNext® End Repair Module: $85. 20 reactions
    NEBNext® Q5® Hot Start HiFi PCR Master Mix: $90. 50 reactions
    Blunt/TA Ligase Master Mix: $97. 50 reactions
    Total: $272

    Regards,

    Fernando

  • #2
    The NEBNext® End Repair Module is missing the A-tailing component (likely taq ploymerase).

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    • #3
      I might be late to the party but does the NEBNext module no longer include the Blunt/TA Ligase Master Mix? It seems that now the kit contains the enzyme only despite the ONT protocol calling for the mastermix. Very frustrating to spend so much on a "one-stop" kit only to find out I'm missing a key component.

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