Hello,
I'm preparing libraries with Nextera XT. From 40 samples 7 are not alright. They are shorter than the rest of my libraries and I'm unsure...
11 results in 0.0024 seconds.
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Sophie's choice: Random mate-pairs or gel selection in Illumina?
Hi guys!
I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs,...Last edited by Marcela Uliano; 01-08-2015, 07:35 AM. -
"home-made" Tn5 transposase for single-cell RNA-seq
here is our paper on Tn5 transposase production which doesn´t require the purchase of an expensive Nextera kit from Illumina:
http://g... -
Possibility of tagmentation without fragmentation with Nextera XT kit
Hello everyone,
I would like to know if it's possible to do the fragmentation of my cDNA amplicon without any fragmentation using the Nextera... -
MiSeq workflow - tissue to instrument
Our lab is new to NGS and we are in the midst of planning our first sequencing run. Our project involves targeted resequencing of 15 horse genes, about... -
Nextera Kit -- DNA fragments too small
Hello --
I have been working with the Nextera kit for Illumina to create DNA libraries for genome sequencing. I initially used the recommended... -
Exome enrichment, How does Nextera Exome Enrichment compares to TrueseqNimbleAgilent?
I have been trying all the different exome enrichment kits available during the last years.
I am now wondering what the real performance of the... -
Best Polymerase for Long Range PCR followed by Nextera XT kit
Hello, I am new to using Next Generation Sequencing.
I am hoping to do long range PCR (20-30 kb), followed by library prep with a Nextera... -
Nextera DNA library - low yield?
I just ran the Nextera DNA sample prep for the first time, following the protocol with the exception of using a Zymo column instead of the spin plate.... -
Poor data but why?
Hello, we're new to MiSeq and finding it difficult to relate the SAV information to our protocol to see what's going wrong.
Has anyone... -
Did you know you can save ~90% on your Nextera Sample Prep cost by miniaturization?
In a recent work that we did at one of the large East Coast pharmas the sample prep cost for Nextera was reduced from $70 to $1.40/sample. We would be...
