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Alignment of ONT data in a Java program through processBuilder
I have a Java-based program for alignment and variant calling. So far we have been using only illumina reads and relied on Bowtie2, which generated great... -
Technology Applications Highlighted at Nanopore Community Meeting
Oxford Nanopore Technologies (ONT) concluded its community meeting in New York City. The event, which took place December 5 through the 7, highlighted... -
Computer Suggestions for Minion Mk1c
Hi,
We are looking to buy a computer since our IT department figured it'll be easier this way. I was wondering what type of computer system... -
Pooling for Oxford Nanopore Multiplex Sequencing
Good Morning All,
I am trying to sequence a few hard-to-ID Enterobacteria using the Oxford Nanopore Rapid Barcoding Kit (SQK-RBK004) on the...Last edited by hathormaat; 03-16-2022, 07:49 AM. -
Using the rapid sequencing kit for cDNA
We haven't done RNA-seq with the minion yet, but have an extra Rapid Sequencing kit lying around. I am wondering if there's anything "wrong"... -
No Signal from ClearLabs Inherited GridION?
Good Morning All,
Our lab has a Clear Labs automated sequencing system that we have been running with a GridION attached. Recently we've... -
MinIon Prep Kit Selection (Ligation vs. Rapid)
Hey all,
I'm looking to sequence a handful of pure bacterial isolates for WGS and de novo assembly. Our group has decided to go with a combined...Last edited by hathormaat; 10-06-2021, 10:39 AM. -
Using Basic MinION Starter Pack in remote area for soil samples
I am not experienced with metagenomics/metatranscriptomics/soil microbiology/Nanopore technology, so please forgive if this post is misguided
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Postdoctoral Fellow: Algorithms for Long Read Transcript Quantification
Long read sequencing has the promise to overcome some of the key limitations of short read sequencing technologies. Among others, long read sequencing... -
Assembly using Illumina + Nanopore 1D reads?
Hi,
I am trying to assemble a eukaryotic genome of about 300MB. I have Illumina data, and I am thinking of trying out the MinION Basic... -
AlignQC - Long-read alignment quality reporting software
Hello all, I haven't started many threads so please redirect me to the right place if I'm not supposed to self-promote here, or there is a tools introduction... -
Nanopolish without reference genome
Hi all!
I am trying to correct raw MinION sequencing reads with nanopolish since nanocorrect seems to be deprecated.
However, during the... -
Experience about Oxford Nanopore sequencing technology
Has been someone using Oxford Nanopore Technology and could tell me about his/her experience?
I am interested in purchase a MiniON equipment... -
Oxford Metrics
Hi,
I am starting a huge oxford project that will generate a huge amount of nanopore sequencing runs. I have yet to start but in my preparations... -
Post-doc Fellow, Third-generation Sequencing & Non-traditional Epigenomics (New York)
In Brief: Epigenomics, PacBio, Oxford Nanopore, RNA Sequencing, Systems/Network biology of infectious diseases and genetic disorders.
Job...
