Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how...
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Calculate number of reads per sample for ddRADseq
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Calculate number of reads per sample for ddRADseq
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how... -
Illumina Sample sheet ddRAD
I have produced a ddRAD library following Poland et al. 2012 . Can anyone help me set up the sample sheet for a mi-seq? I only have one PCR product but... -
pstacks software error
Hello everyone,
can you please tell me how to resolve it .
as i am working on Stacks tool here i am trying to run pstack , cstack... -
GBS:stakcs software error in ref_map.pl
Hello Everyone,
I am currently analysing GBS data using stacks software as i ma currently facing some problem with ref_map.pl.
... -
Data Clean & Adapter Contamination
Hello All,
I am currently starting to analyse my RAD data set (Illumina HiSeq, 150bp PE sequencing run). I have used Trimmomatic for the... -
RAD-seq adapter design
Hi
I was planning a RAD-seq experiment for about 90 ecotypes. The costs of ordering 180+ HPLC purified and modified oligos for those many...Last edited by flobpf; 06-02-2014, 09:08 AM. -
Research Technician Position at the Okinawa Institute of Science and Technology
The Ecology and Evolution Unit (internal: http://ecoevo.unit.oist.jp/ external: https://groups.oist.jp/evolution) at the Okinawa Institute of Science... -
Failed Illumina lane
Hi everyone
Our group has recently sequenced 8 RADtag libraries on Illumina HiSeq 2500.
7 of the lanes were very good but one of them was... -
help! RAD, Stacks process_radtags.pl script, ambiguous RADs
I produced a single digest RAD library using Sbf-I, a rare cutter, following a variation of the Etter (2011) protocol available on Michael Miller's website... -
Restriction-site Associated (RAD) services
Does anyone know of any other companies than Floragenex that perform RAD-tag library production and genotyping? I'm working on sole source justification.... -
2b-RAD? does anyone test it?
Hi all!
Does anyone try to set up a similar experiment?
I'm planning to use 96 tags. Does someone have suggestions about barcodes design?... -
Are RAD data strand-specific?
I think RAD data are not strand-specific, we can sequence both the two strand of DNA, but I found ustacks do regard one sequence and it's reverse complementary... -
Why RAD reads depth vary extremely？
I have got a RAD data set and assessed it. It seems the depth of RAD fragment vary differently, extremely different.
the reads(extract... -
RAD-seq SNPs: pooled population library prep?
G'day Everyone,
I am new to genomics and hope that I don't annoy you by asking a basic question.
I am thinking of using RAD-seq...
