Hi all,
I'd like to share a modular and transparent bash-based pipeline I’ve developed for pre-processing ddRADseq Illumina paired-end...
21 results in 0.0029 seconds.
-
Lightweight bash pipeline for ddRADseq read pre-processing, demultiplexing, and de-duplication using cutadapt
Last edited by rafalwoycicki; 04-23-2025, 06:10 AM. -
Calculate number of reads per sample for ddRADseq
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how... -
Calculate number of reads per sample for ddRADseq
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how... -
mapping hundreds of RADseq fastqs to ref genome.
I would like to map hundreds of fastq.gz single-end RADseq files to a single reference genome.
But, bwa mem is making a map file (.sam)... -
Samples artificially grouped by i5 inline barcodes, in Illumina HiSeq 2500
Hi,
I am using single digest RAD-Seq for analysing the population structure of a protist species, using the dual barcoding by i7 index barcodes... -
Illumina Sample sheet ddRAD
I have produced a ddRAD library following Poland et al. 2012 . Can anyone help me set up the sample sheet for a mi-seq? I only have one PCR product but... -
RAD-Seq Library Loading Concentration?
Hello,
Would I be correct in assuming that RAD-Seq libraries should be treated as if they were low-diversity amplicon libraries (i.e.... -
NGS DNA Library Purification via Dynabeads
Hello, I could use some help from the community.
I have built 48 RAD-Seq libraries using a protocol from Tin et al. (2014) designed for... -
How can I filter out reference sequences that get multiple hits in BWA MEM ?
Hi everyone !
I have exported a small number (~500) of 145 pb sequences from a Stacks analyse.
I then used it as my "reference... -
Building a genetic map from RAD-seq using OneMap
Dear all,
I have some RAD-seq data from 2 parents and 97 offsprings. I would like to use this data to cluster my genomic scaffolds into... -
Genetic map with RAD-seq data
NOTE: I tried to explain my question better here: http://seqanswers.com/forums/showthread.php?t=65681
Dear all,
I have some...Last edited by standonn; 01-20-2016, 05:31 AM. -
bwa mem - paired reads
Hi,
I'm still a fair NGS newbie & I'm trying to run bwa mem (v0.7.9a) on some 91bp paired end sequences & get the following encouraging... -
Hello
Hello,
I'm new to both the site and analysis of NGS data. I'm currently working on analyzing multiplexed RADseq data (Illumina hi-seq)... -
RAD-seq adapter design
Hi
I was planning a RAD-seq experiment for about 90 ecotypes. The costs of ordering 180+ HPLC purified and modified oligos for those many...Last edited by flobpf; 06-02-2014, 09:08 AM. -
Stampy - Mapping multiple individuals to genome
Stampy - Mapping multiple individuals to genome
Hi everybody,
I am very very new to this world, so apologize if the following questions...Last edited by vivi7; 03-18-2014, 12:39 AM.
