Has anyone experienced seeing a prominent non-rRNA peak after rRNA-depletion? I am seeing this consistently in my samples.
See the attached...
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Prominent non-rRNA Bioanalyzer peak after rRNA depletion
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strong 140bp peak following RNA library construction
Hello all,
I have created an illumina truseq library on bacteria by first performing ribozero rRNA depletion kit and then performing a truseq... -
Low gene count assignment for ribo-depleted RNA-Seq data
I originally posted this question on biostars but received little response. I'll be sure to update either post if I receive any more detail.
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Issues with ribo-depletion vs poly-A sequencing in brain tissues
I would like to do an RNAseq comparison between the hippocampus and striatum during contextual fear conditioning. My main interest is to see differential... -
RiboZero kits
Hello,
After looking on the web, I haven't been really successful in finding information about the difference among the several rRNA removal... -
a lot of multiple alignment reads after rRNA depletion
I met the situation of high % reads with multiple alignment sites. Our procedure is removing rRNA by Ribozero kit from ~1 microgram RNA isolated from... -
Ribo-zero - FastQC plots - Per sequence GC content
Dear all
Running Ribo-zero kits on human data, seen consistent FastQC plots like this.
Looks like a few very highly expressed ncRNAs,... -
Combining Ribozero and PolyA data
Hi everyone,
I performed mapping and differential expression analysis of PolyA selected RNASeq libraries and now I'm going to get more... -
degraded clinical RNA samples - to seq or not
Hi,
We have received some human clinical RNA samples. We intend to seq them (Illumina HiSeq) to look for changes in gene expression (protein-coding... -
ribosomal RNA depletion
Hello,
I'm using Epicentre Ribo-Zero kit for removal of rRNA from Drosophila. I checked RNA on Agilent, before and after depletion, and I observed... -
96-sample RNAseq: nextera, hiseq, ribo-zero
Hi,
My lab are new to next-gen sequencing and we're planning an RNAseq experiment with a bunch of E. coli samples and different time-points.... -
DSN-lite, RNase H and Ribo-Zero cook off
I'm curious, has anyone tried the RNAse H method? An elegant method, but you'd have to construct a lot of oligos.
http://www.nature.c... -
C.elegans rRNA reduction kits
I'm wondering if anyone out there has tried/compared multiple rRNA subtraction methods of C. elegans total RNA or has any comments on the matter?
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Mapping statistics from fragmented FFPE samples using Ribo-Zero
Recently we have performed RNA sequencing using Ribo-Zero in order to remove rRNA from fragmented FFPE samples. The number of reads passing filter are... -
Globin & rRNA removal from blood RNA
The new Globin-Zero™ Gold Kit from Epicentre effectively removes the highly abundant adult globin mRNAs and the cytoplasmic and mitochondrial rRNAs from...
