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I'm curious, has anyone tried the RNAse H method? An elegant method, but you'd have to construct a lot of oligos.
- Genohub
- Genohub
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I've been playing around with the RNase H method, but it's proven a bit finicky for my samples, at least as far as the Bioanalyzer traces show. When it works, it really seems to work well, but I've also had a few test samples become completely degraded.
The method is patented, so I'm hoping that a commercial kit will be put out that supplies the oligos and full-proofs the method a bit more.
Very promising stuff though. -
Keep me updated if you ever attempt to sequence your RNase H samples. Did you design all the oligos yourself? I'm interested in designing some for mtRNA removal. Yes, a commercial kit would be nice. Anyone know of one coming?
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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