12 results in 0.0023 seconds.
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RNA Extraction from Frozen EDTA Blood
Is there anyone who can isolate RNA from frozen EDTA blood sample or develop a method? I am open to all kits and methods on this subject. -
RNA purification, DNA contamination, and small RNA sizing profiles
Hello all,
I am extracting total RNA from tissues derived from wild animals after 24-48 h of death. I am able to get a RIN of approximately... -
Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extracti
Hi All,
I was thinking this might be interesting for some doing RNA/DNA sequencing. We have established home-made methods for HT RNA/DNA extraction... -
How to define RNA quality for Drosophila RNA using Agilent Bioanalyzer?
Hi all,
I extracted RNA from Drosophila heads, and want to do RNAseq on them. I used Agilent's Bioanalyzer to assess RNA quality, and found... -
Hello
Hi everyone
I'm new to the forum and quite new to RNA work too.
I would like to know if anyone has experience isolating RNA from saliva... -
How to deplete RNA without RiboZero or Terminator exonuclease?
Hello everyone!
Thank you for reading my question! Please excuse my little experience in the topic, I would like to have some advice on the RNA-seq... -
How much DNA contamination "allowed"?
Hi,
We're going to do a RNA-SEQ experiment (with Nextseq 500) with bacterial RNA for differential gene expression. We have cells lysed... -
Speed vac concentration of RNA?
Hi, I have many RNA samples extracted using Ambion Mirvana which I am planning to hybridise to Affymetrix HTA arrays. The protocol requires 150ng RNA... -
trizol + RNeasy tot RNA extractions: decrease trend in last samples concentration
Hi there
I have done several (about 800) tot RNA extractions using trizol followed by RNeasy clean-up.
I usually do 20 samples per... -
Total RNA stability following extraction/isolation
Hi all,
I will be extracting total RNA from animal tissue using the RNeasy minikit. The final step of which is to elute in to RNase-free... -
RNA-seq libraries from bird blood
Hi all,
I am planning a RNASeq experiment and I would like to prepare some Illumina libraries. The starting material is avian blood. I had a look... -
RNA extraction from lizard
Hello all
I want to sequence the transcriptome of a new organism (species of lizard)
i order to do that, i need first to...
