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Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extracti
Hi All,
I was thinking this might be interesting for some doing RNA/DNA sequencing. We have established home-made methods for HT RNA/DNA extraction...
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Hello
Hi everyone
I'm new to the forum and quite new to RNA work too.
I would like to know if anyone has experience isolating RNA from saliva...
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How much DNA contamination "allowed"?
Hi,
We're going to do a RNA-SEQ experiment (with Nextseq 500) with bacterial RNA for differential gene expression. We have cells lysed...
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Speed vac concentration of RNA?
Hi, I have many RNA samples extracted using Ambion Mirvana which I am planning to hybridise to Affymetrix HTA arrays. The protocol requires 150ng RNA...
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trizol + RNeasy tot RNA extractions: decrease trend in last samples concentration
Hi there
I have done several (about 800) tot RNA extractions using trizol followed by RNeasy clean-up.
I usually do 20 samples per...
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Total RNA stability following extraction/isolation
Hi all,
I will be extracting total RNA from animal tissue using the RNeasy minikit. The final step of which is to elute in to RNase-free...
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RNA-seq libraries from bird blood
Hi all,
I am planning a RNASeq experiment and I would like to prepare some Illumina libraries. The starting material is avian blood. I had a look...
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RNA extraction from lizard
Hello all
I want to sequence the transcriptome of a new organism (species of lizard)
i order to do that, i need first to...