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  • Dampor
    Member
    • Jun 2012
    • 14
    #1

    trizol + RNeasy tot RNA extractions: decrease trend in last samples concentration

    Hi there

    I have done several (about 800) tot RNA extractions using trizol followed by RNeasy clean-up.
    I usually do 20 samples per day. Although I am generally satisfied with what I get, especially in terms of quality, I notice that last samples of my daily extraction course are always less concentrated than the first ones.

    Do anyone know why this happens?

    Cheers
    Dam
    Tags: rna concentration, rna extraction, rneasy, trizol
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