Hi,
I am very new to RNA-seq. I have tried making libraries from 5 samples: 3 from eukaryotic RNA and 2 from bacterial RNA. The starting...
6 results in 0.0023 seconds.
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SriptSeq library preparation
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RNase III digestion time for Ion Total RNA-seq
We are doing poly A selection from 100ng of Total RNA. After building the libraries, the insert size looks to be around 100bp. We are wondering if the... -
RNA fragmentation
Hello all,
I am new to here and RNA-Seq. So I need some help.
I am working on optimizing the RNA fragmentation conditions... -
RNA fragmentation x cDNA fragmetation biases
Hello,
I was reading "Wang et al, 2009: RNA-seq, a revolutionary tool for transcriptomics" and I have some doubts:
... -
DNase treatment and fragmentation of RNA
Hi all,
I have recently started RNA extraction on different fish tissues with the aim of carrying out RNA-seq using Illumina Hi-seq.... -
mRNA fragmentation for 100bp paired-end reads
Hi everybody,
I am currently planning a mRNA-seq experiment in a non-model species with the first goal being to study multiple copies elements...Last edited by amdic2; 08-29-2011, 05:50 AM.
