RNA fragmentation x cDNA fragmetation biases
Hello,
I was reading "Wang et al, 2009: RNA-seq, a revolutionary tool for transcriptomics" and I have some doubts:
Someone could tell when should we use/choose the RNA fragmentation or cDNA fragmentation method for RNA-seq library? Do they have differences in applications?
What are the specifics factors in RNA fragmentation that causes the "gene body" biases? And what are the specifics factors in cDNA fragmentation that causes the 3´end biases? or Why ?!!
I have seen several similar post, but I have not been able to find the answers!!
Thank you very much
I was reading "Wang et al, 2009: RNA-seq, a revolutionary tool for transcriptomics" and I have some doubts:
Someone could tell when should we use/choose the RNA fragmentation or cDNA fragmentation method for RNA-seq library? Do they have differences in applications?
What are the specifics factors in RNA fragmentation that causes the "gene body" biases? And what are the specifics factors in cDNA fragmentation that causes the 3´end biases? or Why ?!!
I have seen several similar post, but I have not been able to find the answers!!
Thank you very much