Hi everyone,
I'm new here and my searches didn't quite give me what I wanted so hopefully this isn't an already answered question. Any help is...
18 results in 0.0022 seconds.
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Extract rRNA from RNASeq data
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inexplicable rRNA content
Hi guys,
long time reader, first time poster here. So here's my problem:
Did two RNAseq runs (NextSeq500, RNA Access Illumina... -
How to get the number of rRNAs in your genome
I have the NGS genome data of an e.coli species and I wanted to know the number of rRNAs present in the genome. Which tool can I use? -
Cannot find 18s and 28s ribosomal RNAs in the GTF file from UCSC
Hello everyone,
I couldn't find 18s and 28s rRNA's in the GTF file from UCSC genome browser. I want to map against hg19/GRCh37 genome.... -
Ribosomal depletion for RNA seq
We are currently embarking on an RNAseq project using a sequencing service provider but have a few questions concerning the ribosomal RNA depletion step... -
SeqMonk and featureCounts disagree on rRNA
This is actually a follow-up from a previous problem I had described in this post.
In this experiment we've taken cultured human cells,... -
Where can I find rRNA databases?
The only rRNA database I know is SILVA, but it only contains 16S/18S and 23S/28S rRNAs
What are some other rRNA database suggestions? -
rRNA contamination due to GL000220.1
We are carrying out RNAseq on some human tissues and have been facing rRNA contamination issues.
We first carried out library prep with the Ribozero... -
(Problem) Prediction rRNA with rnammer 1.2
Hi,
I tried to predict the rRNA in scaffold. (de novo assembly genome)
My sample is human genome and I set the euk with rnammer param.... -
High %rRNA in NuGen Encore invert libraries
We recently did a number of library generations using the Nugen Encore kit. The samples were from flies, Drosophila melanogaster; extractions and library... -
Can someone explain how Ribo Zero works?
I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant... -
Low RIN of plant RNA and high abundaces of rRNA.
Hi there and thanks in advance for your input!
We extracted total RNA from (plant) leaves, using the quiagen RNeasy kit. As expected, we... -
rRNA identification tools or information PLEASE!
Hi everybody,
I identified in my genome (by blast) 1 contig which is the rRNA genome.
Now, I am searching for tools able... -
Ribozero Output
Hi all,
I have some rRNA which was filtered out of my total RNA sample using Ribopicker. My sequence data is paired ends. I was wondering... -
% rRNA remaining after polyA selection
Does anyone have a standard or threshold of % rRNA they require in order to proceed with mRNA library prep?
In the past I've only proceeded...
