My question is that during the SMART-seq3 testing for single-cell RNA sequencing, after reverse transcription and PCR, the products either...
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"single cell" "SMART-seq3" "cDNA library" "sharp peak" and “byproduct”
Hi，All
My question is that during the SMART-seq3 testing for single-cell RNA sequencing, after reverse transcription and PCR, the products either... -
sharp peak in library - TruSeq Stranded Total RNA (rRNA-depleted E. coli)
Anyone seen anything like the sharp peaks at 250 bp in the bioanalyzer traces of the libraries below? Overamplified? Bubble product?
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Truseq cDNA libraries show sharp peak of cDNA
Hi everyone! I just completed cDNA library prep using Illumina's Truseq RNA sample prep v2. Out of 8 libraries, 6 show the appropriate size distribution...
