Hi everyone! I just completed cDNA library prep using Illumina's Truseq RNA sample prep v2. Out of 8 libraries, 6 show the appropriate size distribution of my cDNA fragments (around 200-500bp). However, the first and sixth sample show a much sharper peak of cDNA fragments, right about 200-250bps. (An agarose gel is attached- the last lane is an older cDNA library I ran as a control). qPCR using adapter specific primers shows that these fragments do have ligated adapters. What could have caused this sharper peak? And are these samples comparable if I submit for sequencing, or should I repeat the library prep?
Thanks!
Thanks!
Comment