"single cell" "SMART-seq3" "cDNA library" "sharp peak" and “byproduct”

gao gao

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Join Date: Dec 2024

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"single cell" "SMART-seq3" "cDNA library" "sharp peak" and “byproduct”

12-25-2024, 07:11 AM

Hi，All
My question is that during the SMART-seq3 testing for single-cell RNA sequencing, after reverse transcription and PCR, the products either show wide peaks of byproducts, or in the 1000-2000 bp range, I am unable to obtain a smooth peak, as there are always many sharp peaks. Has anyone attempted to eliminate these?

just like B AND C

thanks
gao gao
24.12.25
Tags: sharp peak, single cell rna seq, smart-seq3

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