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SMART-seq2 vs SMART-seq3/FLASH-seq RT/TSO primers and cDNA amplification
Can anyone offer any insight why in SMART-seq2 the RT and TSO primers are designed in a way that cDNA is amplified by semi-suppressive PCR (suggesting... -
Downloading smart-seq2 fastq files from NCBI GEO
Hi,
From what I understand, for smart-seq2 sequencing files there are multiple fastq files per sample with one single/pair per cell. However,...Last edited by malonzm1; 05-08-2023, 07:42 PM. -
Vapor-Lock/Mineral oil for Smart-seq2 miniaturization?
Dear community,
My name is Jakob Theorell and I am currently a post doc at the University of Oxford. I have just started running Smart-seq2 for... -
RNA-seq read alignment best practices for low-input Smart-seq2 samples
RNA-seq read alignment best practices for low-input Smart-seq2 samples
Hi All,
I think my data may fall in a nether region between... -
Single-cell transcriptomic: rare transcripts
Hi!
I performed a smart-seq2 experiment on B-cells. After some minor changes in the protocol I got a satisfying amplification with no primer... -
UMI in Smart-seq2 protocol
Hi All,
Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account... -
Variance in cDNA amplification
Hello,
I recently generated cDNA from 100 T-cells (from the same source) using the Smart-Seq2 protocol from Sandberg et al. After the pre-amplification... -
Smart-seq2 sample prep questions
Hi All,
I have a couple questions about the Smart-seq2 method for amplifying single cells (Sandberg 2014), and I hope someone with experience...
