Hi,
I'm currently trying to use the CogentAP pipeline to process some bulk rna seq from murine sorted cells. We used the SMARTer Stranded...
6 results in 0.0042 seconds.
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Illumina Experimental Manager - SMARTer sample prep kits txt
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Hi all, newbie LCM-RNAseq user here
I am interested in finding out the best methods to sequence RNA from laser capture micro dissected (LCM) mouse brain tissue. I will have low quantity... -
Clontech Low Input Library Prep Kit
Hello,
Anyone used Clontech Low Input Library Prep Kit (Cat. No. 634947)? It is suggested for the lib prep following cDNA synthesis/amplification... -
DSN-lite, RNase H and Ribo-Zero cook off
I'm curious, has anyone tried the RNAse H method? An elegant method, but you'd have to construct a lot of oligos.
http://www.nature.c... -
cDNA purification prior to shearing (for Illumina sequencing)
Hi!
I recently got the SMARTer Ultra Low RNA Kit for Illumina Sequencing, and I'm confused about their way to purify ss- and ds cDNA (they... -
why rG and not dG in the template switch oligos (TSO) in single cell library preps?
Hi all,
I am preparing single cell libraries for RNAseq using the SMARTer II kit (Clontech) and I was wondering whether there is a reason why they...
