Hi,
Could I use a Branson2800 sonicator to fragment genomic DNA (350pb)?
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Branson sonicator
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Questions regarding ChIP sonication and ChIP-Seq on heterochromatin
Hi all. I have a problem regarding ChIP sonication. We are culturing one type of cells in two different states. One state shows active transcription (say... -
Covaris M220 service plan
Hi all,
Our department is considering purchasing a Covaris M220 so we can begin generating libraries in house. A one year service plan... -
library prep using sonicator - indexing
I'm currently using Nextera XT to prepare libraries for MiSeq. This involves 'tagmentation' which simultaneously fragments (enzymatically) and adds a... -
AT rich region depletion
We are having some trouble with our ChIP-seqs: we seem to be completely blind to AT rich regions. When I check the GC distribution in the ChIP and Input... -
Incomplete shearing with Bioruptor?
Hi all!
Shearing gDNA for NGS sample prep. I'm trying to shear my DNA into 500bp fragments using the bioruptor and have two problems: ... -
Bioruptor (serious) decreasing efficiency overtime
Dear mates,
I've been extensively using the Bioruptor for shearing chromatin for ChIP applications. I came to use different machines of... -
maximum size sonicated DNA?
What is the maximum DNA size people can reliably obtain when sonicating genomic DNA? I've seen papers that claim they can sonicate to 2-5Kb and then do... -
What is at the ends of sonicated DNA?
Given the poor molar yields of typical next gen library construction methods, I have often wondered whether end-repair after shearing was an issue. There... -
High-Throughput DNA Shearing
Hello,
Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously?...Last edited by ABSOLiDuser; 03-09-2009, 07:35 PM.
