Hello!
I am currently using RNA-seq to examine the roles of a few key proteins in the transcriptional response underlying a certain condition....
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Bayesian network analysis to infer gene regulatory relationships
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Transcription Factor Binding Sites, Motifs and Profiles from ChIP-seq data
ChIPBase v2.0 is designed for studying transcription factor binding sites and motifs, and decoding transcriptional regulatory networks of non-coding RNAs... -
Identify TFs in New Assembled Organism
A denovo assembly of a new organism that lives in the sea (from the animal kingdom but we do not know its type specifically) have been prepared.
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Get DNA Binding Domains of Transcription Factors
My question is basic and as follows:
I have 1000 transcription factors (TFs) which I have retrieved their amino acid sequences from Uniprot.... -
Anyone using Bioruptor Pico?
Hi,
I'm wondering whether anyone here has ever succeeded using Bioruptor Pico sonicator for transcription factor ChIP-qPCR.
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Find binding sites for transcription factor in a sequence
I want to look at the promoter sequence of human IgM (ENSG00000162897), which I can easily retrieve with biomart. I'm specifically looking for potential... -
Transcription factor interactions via multiple motif discovery
Hi All,
I have a high confidence ChipSeq data set for TF1 and have run MEME to discover the most likely canonical motif (motif1), which... -
5' promoter/alignment question
I am looking at TFBS analysis for a colleague. He is interested in TRANSFAC but it being commercial I am interested in JASPAR. From Wasserman and Sandelin... -
ChIPseq on tissue - eliminating background
Hi,
I am trying to do ChIPseq on tissue for a transcription factor that is expressed in a single cell type within a tissue. Therefore,... -
deepCAGE in expression quantification and modeling
Nature Genetics
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line.
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