Tweet
Hi, I fed a specific custom adapter sequence to trim galore on paired-end illumina files (~3B bp each), and it removed approx. 7M bases from each (--length cut-off and --quality both set to 0) . However, when I searched for direct string matches (using grep) on the fastq files, it showed no matches at all. Why did that happen? Perhaps it has to do it trim-galore's approach being more complex than simple lookup-and-cut?
-
Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM