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  • Try_101
    Junior Member
    • Feb 2021
    • 1

    Deblur, Unknown variant, unable to interpret PHRED

    Hey guys,

    I am having trouble using deblur --worflow on all of my fastq data.

    when using --workflow, with just an fastq input and trim I allways get the same error message

    Code:
    Unknown variant, unable to interpret PHRED
    even when I made a Simple file with just

    @SEQ_ID
    GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACA
    +
    !''*((((***+))%%%++)(%%%%).1***+*''))**55CCF>>>>>>CCCCC

    I still get the same Error when I use Deblur.
    I am using Linux Debian and installed Deblur using a conda env.

    Does anybody know, how I can fix this issue? Im totally out of Ideas.

    Thank You

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