17 results in 0.0217 seconds.
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Effect of Over-Represented Transcripts on DEG Analysis
We have a fairly simple set of RNA-seq data that we are using to perform a DEG analysis, breifly, a wild-type strain and a single gene knock out (in yeast).... -
fastqc shows strange GC content
Hi,
we have a 36-samples ChIP-Seq experiment with a very strange GC-content behavior (s. images)
The samples are from yeast. This... -
library depths for sequencing
Hi everybody,
I was approached by a biologist in our lab and was ask a specific question. As I was not really sure, I would like to share... -
PhD scholarship in yeast evolutionary genomics in Auckland, New Zealand
We are seeking an enthusiastic PhD student to join the Institute of Natural and Mathematical Sciences at Massey University, Auckland, New Zealand. ... -
rRNA Yeast
Hi all.
I am searching for complete list of rRNA's sequence of Yeast but I didn't find it. Could anyone help me?
Thank you in advance... -
Next gen sequencing analysis - for small genomes
Hi,
I am working on an organism with a small genome (Toxoplasma gondii, 65 Mbp). With ChIP-seq, we naturally tend to get very high sequence... -
Assemblers for Short Reads
I want to use an assembler to assemble the unmapped illumina reads from an alignment to S288C (yeast genome). So far I have been using SSAKE and it seems... -
PCR-free; absolute abundance; yeast
I am new to NGS.
Goal is to find absolute abundance of yeast species in a mixed sample.
I plan to use PCR-free methods since PCR could cause... -
Bowtie question about yeast SK1
Hello all,
As many of the posts on the site indicate, I am new to the world of bioinformatics. I want to do alignment of my ChIP-seq data to the... -
de novo assembly with MIRA and 454 single-end reads. Too much contigs
Hi everybody!
I'm new in NGS data processing and also in this great community.
I have two sets of reads 454 single-end from Roche... -
Continuous AF with GATK
Hi,
I saw some VCF files that have a continuous AF value. In my results the AF values are either 1 or 0.5.
I am working on yeast, so the... -
Recommendations for yeast mutation identification
Hi
I am just starting a new project and fishing for what the latest recommendations are for bioinformatic tools or workflows to identify mutations... -
Yeast filter-reference file.
Hi guys,
My apologies if the question is simple or trivial, but I am not an expert in this type of analysis.
I am trying to perform a whole... -
variant annotation for yeast (saccer)
Hi,
We have done WG hiseq sequencing of yeast strains s. cerevisiae
and I wanted to ask if anyone knows a program that can annotate
... -
yeast rRNA contamination: Illumina prep
Our lab is making a library from yeast total RNA. We have tried NuGen kit, and got huge rRNA peaks in the samples. We then tried Illumina's RNA-Seq...
