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  • Gene name assignment/ Merge genome-based assembled transcripts with de novo transcrip

    Dear all,

    I am planninng to build a transcriptome database of a chicken cell line from RNA-seq data. The genome information is available, but it lacks not a few number of gene sequence information. Thus, I'd like to integrate genome-based assembly with de novo assembly. The two data are isoform-aware.

    Here, I have two questions;
    1.Are there any good tools available to merge two set of transcriptome data, generated from genome-based assembly and de novo assembly?
    2.How to assign gene names to the de novo assembled transcripts?


    Thanks in advance for your help!
    Last edited by htk; 04-01-2013, 02:35 AM.

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