hi After running velvet assembly I got Estimated Coverage = 25.12000
Estimated Coverage cutoff = 10.250000
Final graph has 11106 nodes and n50 of 498, max 13308, total 243547, using 221137/2500000 reads, so I have 3 questions here.
1) what does this max 13308 ( It is the maximum contig length obtained)
2)what does this total 243547 refers to?
3) should I run the velvet again by using the obtained estimated cov cutoff and estimated coverege (for eg here should I run velvet again using cov_cutoff 10
est_coverege 25)
can any one answer this???
thanks
Estimated Coverage cutoff = 10.250000
Final graph has 11106 nodes and n50 of 498, max 13308, total 243547, using 221137/2500000 reads, so I have 3 questions here.
1) what does this max 13308 ( It is the maximum contig length obtained)
2)what does this total 243547 refers to?
3) should I run the velvet again by using the obtained estimated cov cutoff and estimated coverege (for eg here should I run velvet again using cov_cutoff 10
est_coverege 25)
can any one answer this???
thanks
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