Hi all,
A few days ago I completed a ChIP experiment from 10^8 cells that yielded a whopping 2.5ug of DNA at the end! The input (whole cell lysate) had about 3.5ug. This experiment has been validated with one of our collaborating labs, as well as myself back in the Fall. Both of us were used to getting 20-50ng total for library preparation. I'm wondering if one of the reagents went bad, such as the BSA solution for blocking, or any ideas about what could have happened. That much DNA just seems way too suspicious. Thanks!!
I will repeat this experiment regardless but would rather make an educated guess before going through more cells and reagents.
A few days ago I completed a ChIP experiment from 10^8 cells that yielded a whopping 2.5ug of DNA at the end! The input (whole cell lysate) had about 3.5ug. This experiment has been validated with one of our collaborating labs, as well as myself back in the Fall. Both of us were used to getting 20-50ng total for library preparation. I'm wondering if one of the reagents went bad, such as the BSA solution for blocking, or any ideas about what could have happened. That much DNA just seems way too suspicious. Thanks!!
I will repeat this experiment regardless but would rather make an educated guess before going through more cells and reagents.
Comment