Hi, sorry for bothering you guys. I am using the agilent Sureselect methyl-seq target enrichment kit to generate library for illumina sequencer (high seq). after bisulfite treatment, I ran a QPCR to quantify how much DNA I got and only got about 20 ul 100 pM product, less than the expected concentration, 200 pM. I followed agilent protocol exactly. is any one using this kit and can give me some suggestion to improve the amount of product captured by this kit?
thank you so much in advance!!
ding
thank you so much in advance!!
ding
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