ChIP reagents

[ChIP Kick]

Hi, I tried many times but I still cannot get a good sonication condition for my exp. Now I used all my lysis buffer which I bought from Millipore(EZ-Magna ChIP™ A Chromatin Immunoprecipitation Kit). I checked their web but no recipe for it.There are so many different recipes for lysis buffer. I do not know which one is good for my exp.

So is there someone who could give me suggestions to make my own cell lysis buffer and nuclear lysis buffer?

Thanks a lot!

[kwaraska]

Do you have access to any Coveris Instrument? They have a very good ChIP kit which has worked well for most of my customers.

I would suggest contacting Covaris (www.covarisinc.com) and finding out if they have an instrument in the area....

Unless of course you are in Boston-then you can use ours.

[ChIP Kick]

Thanks! Unfortunately we don't have Coveris Instrument. I can fly to Boston to use yours...I am just kidding

I still need to make my own cell lysis buffer and nuclear lysis buffer? Anyone can help? Thank you in advance!

[epigeneticfan]

Diagenode has the highest citation ranking for chromatin shearing (700+ papers). Diagenode has also developed specific chromatin shearing optimization modules. These *modules are compatible with Chromatin Immunoprecipitaions kits from Diagenode. The choice between modules is based on your experimental requirement: number of cells to be used in ChIP, down-stream application (qPCR analysis or ChIP-seq experiment). For example, Chromatin Shearing Optimization kit-Low SDS is recommended for ChIP-seq assay resulting in the *superior performance ( high enrichment *with low signal-to-noise background). Moreover, Diagenode is not exclusively proposing shearing optimization kits (like Covaris does) but proven and validated fully integrated ChIP kit solutions as well as a sonication device Bioruptor (more than 700 citations). *Of course, further optimization might be required for chromatin depending on cell type. The techsupport team is ready to extend its help for any specific request and also a demo installation of the Bioruptor can be organized.
More about Diagenode Chromatin Shearing can be found at **http://www.diagenode.com/media/docum...r_protocol.pdf