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  • DonDolowy
    Member
    • Oct 2012
    • 56
    #1

    MeDIP-seq and compatible DNA adapters for multiplexing?

    Dear all,

    I am about to run MeDIP-seq on several samples and are looking for compatible DNA adapters for PE multiplexing (probably 2-plexing) on a HiSeq2000.

    Apparently, Illumina´s TruSeq adapters are incompatible since they are methylated. Do you know if NEBnext´s adapters (E7500) are compatible? Or can you recommend something else?

    I am following the protocols from Taiwo et al, 2012 and Ethan Ford (Online version from Ethanomics).

    Thanks a lot!

    Best,

    Kevin
    Tags: None
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