Hello everyone,
My supervisor and I are working with bisulfite-treated whole genome sequencing data but we're having issues understanding the nuances of directional vs. non-directional libraries.
Specifically, in the Lister/Ecker paper published in 2009 detailing the MethylC-seq pipeline for analysing bisulfite-treated data (Finding the fifth base: Genome-wide sequencing of cytosine methylation), did they use a directional or non-directional library? Nothing in the paper seems to indicate which one was used.
Thanks,
Daniel
My supervisor and I are working with bisulfite-treated whole genome sequencing data but we're having issues understanding the nuances of directional vs. non-directional libraries.
Specifically, in the Lister/Ecker paper published in 2009 detailing the MethylC-seq pipeline for analysing bisulfite-treated data (Finding the fifth base: Genome-wide sequencing of cytosine methylation), did they use a directional or non-directional library? Nothing in the paper seems to indicate which one was used.
Thanks,
Daniel
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