Hi,
Is there anyone else out there who are using the MiSeq (or HiSeq) platform for performing paired end whole genome bisulphite (aka bisulfite) sequencing? I'd like something to compare my QC reports to to determine whether it is as expected or not.
For example (after trimming, etc):
I have a hump of read lengths around 75-100 which drops off before the main peak of read lengths of the full read length of 260.
The quality distribution is U-shaped from phred-20 to phred-40
The nucleotide contributions for each base position are bowed.
*Using EpiTect Bisulfite Kit & EpiGnome Methyl-Seq Kit*
Cheers
Is there anyone else out there who are using the MiSeq (or HiSeq) platform for performing paired end whole genome bisulphite (aka bisulfite) sequencing? I'd like something to compare my QC reports to to determine whether it is as expected or not.
For example (after trimming, etc):
I have a hump of read lengths around 75-100 which drops off before the main peak of read lengths of the full read length of 260.
The quality distribution is U-shaped from phred-20 to phred-40
The nucleotide contributions for each base position are bowed.
*Using EpiTect Bisulfite Kit & EpiGnome Methyl-Seq Kit*
Cheers
Comment