Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • aniruddha.otago
    Member
    • Jan 2010
    • 21
    #1

    Help with bisulfite sequencing

    Hi,
    We are daling bisulphite sequencing and have send DNA samples now for sequencing to illumina/solexa. can any one tell me how can i deal with the data after sequencing. my study is on DNA methylation. what may be the starting point. because i really dont know anything about it. can anyone help.
    [email protected]. please...
    Tags: None
  • simonandrews
    Simon Andrews
    • May 2009
    • 870
    #2
    Originally posted by aniruddha.otago View Post
    Hi,
    We are daling bisulphite sequencing and have send DNA samples now for sequencing to illumina/solexa. can any one tell me how can i deal with the data after sequencing. my study is on DNA methylation. what may be the starting point. because i really dont know anything about it. can anyone help.
    [email protected]. please...
    You'd probably be better putting this in a new thread rather than adding it to the end of this one.

    There have been a couple of threads already about this if you search back through old posts but it might be worth going through it again.

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2
    Started by SEQadmin2, Yesterday, 11:08 AM
    0 responses
    6 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    Yesterday, 11:08 AM  
    New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    11 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-30-2026, 05:37 AM  
    Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    19 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-26-2026, 11:10 AM  
    Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    53 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-17-2026, 06:09 AM  
    View All
    Working...