ChIP-seq library complexity

zwang0614

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Join Date: Feb 2014

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#1

ChIP-seq library complexity

02-14-2014, 12:46 AM

Hi all.
I am new to the ChIP-seq and trying to ChIP genome-wide H3K27me3. Is there any methods or strategies for increasing the library complexity? Maybe shearing more using sonicator is one, but I am worrying about more sonication will hurt the K27 methylation and lead to poor IP. Or should I use more starting materials? Any thoughts would be grateful!

Wang
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NextGenSeq

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Join Date: Apr 2009

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#2

02-14-2014, 10:45 AM

Make sure you don't overamplify your library. We always aim for a final library concentration of 10 to 20 ng/ul. If your yield is higher than that you are overamplifying.
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