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  • zwang0614
    Member
    • Feb 2014
    • 10
    #1

    ChIP-seq library complexity

    Hi all.
    I am new to the ChIP-seq and trying to ChIP genome-wide H3K27me3. Is there any methods or strategies for increasing the library complexity? Maybe shearing more using sonicator is one, but I am worrying about more sonication will hurt the K27 methylation and lead to poor IP. Or should I use more starting materials? Any thoughts would be grateful!

    Wang
    Tags: None
    • NextGenSeq
      Senior Member
      • Apr 2009
      • 482
      #2
      Make sure you don't overamplify your library. We always aim for a final library concentration of 10 to 20 ng/ul. If your yield is higher than that you are overamplifying.

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