Hands-On Training in DNA Methylation Analysis

[ecSeq Bioinformatics]

DNA Methylation Analysis - How to Use Bisulfite-Treated Sequencing to Find Methylated Regions

When?
October 20th - 21st 2014

Where?
Leipzig, Germany


Scope and Topics
The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained on understand bisulfite-treated NGS data, detect potential problems/errors and finally implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:

• be familiar with the sequencing method of Illumina
• understand how bisulfite sequencing works
• be aware of the mapping problem of bisulfite-treated data
• understand how bisulfite-treated reads are mapped to a reference genome
• be familiar with common data formats and standards
• know relevant tools for data processing
• automate tasks with shell scripting to create reusable data pipelines
• perform basic analyses (call methylated regions, perform basic downstream analyses)
• plot and visualize results (ready-to-publish)
• be able to reuse all analyses

Find more information at http://www.ecseq.com/workshops/workshop_2014-03.html

[ecSeq Bioinformatics]

Combine you methylation workshop one of our other courses and you will get a 10% discount to all booked workshops!