difference between illumina RRBS-seq and HM450 array

[Yehudit]

Hi,
I have a question about the difference between illumina RRBS-seq and HM450 array for methylations sequencing.

I'm trying to understand what is RRBS advantage over HM450, if HM450 is covering 96% of CpG islands and 99% of RefSeq promoteres, while RRBC cover only 85% of CpG island? (And also the price per sample is similar...)

Thanks!

Yehudit

[dpryan]

One is NGS based and the other is an array. Arrays don't yield single base resolution, RRBS does. BTW, one could also just use a capture kit rather than RRBS and likely up the coverage to match the HM450 array.

[Yehudit]

Thanks...

What kind of information do i get from the array?

[dpryan]

You get probe intensities from the array that you then have to convert to a score and then map to a region (i.e., the same thing you get from all arrays). There are tools for processing both arrays and sequencing-based datasets, though the latter is under more development since people don't use arrays much anymore.