I want to use ChIP-seq to find the new target sites of a transcription factor.but i was confused by the sample preparation:excise 100-200bp
dna fragment from gel after ligation and pcr to sequence.
in ChIP,the DNA was sonicated into 500-1000bp,so where were the 100-200bp ragments from?
dna fragment from gel after ligation and pcr to sequence.
in ChIP,the DNA was sonicated into 500-1000bp,so where were the 100-200bp ragments from?
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