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  • lancelothk
    Junior Member
    • Sep 2013
    • 6

    partial methylation/intermediate methylation

    For single CpG site, what may cause the partial methylation/intermediate methylation in BS-seq data?
    What I know are:
    1. allelic methylation difference in diploid cell.
    2. sequencing/bisulfite error (after trim lower quality bps, filter out low bisulfite conversion rate reads)
    3. Inter-cell variation for mix cells sample.

    How to distinguish this from other type?
    Is there any literature about this topic?

    Thanks.

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