Dear everyone
I have just prepared my multiplexed reduced representation bisulfite sequencing library. It seems like I have 4 peaks on my bianalyzer results arround 180, 235, 300 and 360 base pairs.
Have any of you experienced these peaks before?
I do multiplexed RRBS with Msp1 digestion, TruSeq Nano adapter ligation and cleanup with dynabeads AMPure XS.
Myself Im thinking that it could be that the Msp1 has a high cutting efficiency at these peaks. Do you have any suggestions?
Do you think that these libraries is good to go for sequencing?
Kind regards from Emil.
I have just prepared my multiplexed reduced representation bisulfite sequencing library. It seems like I have 4 peaks on my bianalyzer results arround 180, 235, 300 and 360 base pairs.
Have any of you experienced these peaks before?
I do multiplexed RRBS with Msp1 digestion, TruSeq Nano adapter ligation and cleanup with dynabeads AMPure XS.
Myself Im thinking that it could be that the Msp1 has a high cutting efficiency at these peaks. Do you have any suggestions?
Do you think that these libraries is good to go for sequencing?
Kind regards from Emil.
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