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  • reprogrammer
    Member
    • Jan 2016
    • 12

    Downstream analysis of methylkit

    I have finished my RRBS analysis with methylkit R package. It returns me plenty of results, such as methylation distribution, coverage, clustering, annotation in the genome, DMRs and differential methylated genes.

    What could I do next with the data methylkit provided? I would like to show the curves of the methylation levels on upstream +5000 bp, gene body, and downstream -5000 bp. Which pipeline or package can help me do this?

    Thank you very much.

    Jerry

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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