Hello all. I'm exploring ways to sequence Epstein-Barr virus genome (~170kb episomal dsDNA) in a mixture of human DNA. I'm thinking the situation might be similar to that of sequence mtDNA/chloroplast DNA in total DNA. We tried PCR + Illumina GA method and it worked well. But for some of our samples we don't have enough DNA for all PCR reactions. We are thinking abt the WGA + Nimblegen technology before going for NGS. Can anybody tell me what are there pitfalls of such approach? Thanks a lot.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM